Phylogenetic analysis of Streptococcus cristatus isolated from an infected root canal

Abstract

Objectives: This research aimed to investigate the identification by molecular and morphological techniques of a new local strain of Streptococcus cristatus. This strain was detected in 30 cases of patients with infected root canals. Methods: In this study, a modification of Schaedler Agar Medium was done to encourage the isolation of this strain. Then tests of ability for slime layer production and Antibiotic sensitivity were done. Results: The results showed that only three out of the thirteen cases gave positive results for S. cristatus, estimating a 10% prevalence. PCR and gel electrophoresis were employed to further verify this outcome. S. cristatus's 16S rRNA gene sequence was ascertained and compared with the present information from the National Center for Biotechnology Information (NCBI) to determine the strain specifically. The accession number PV243282.1 was assigned to the sequence following its submission to GenBank. This strain was registered in GenBank under the name Mosul. Furthermore, the study confirmed that the isolate under study could create a slime layer and the capacity to produce biofilm. Conclusion. The results of this study enhance the knowledge about S. cristatus and its traits in relation to infected root canals, offering insightful information for future studies and Possible approaches to treatment.